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cd11b enrichment buffer  (Miltenyi Biotec)


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    Miltenyi Biotec cd11b enrichment buffer
    Common antibodies used for analysis of microglial cells by flow cytometry
    Cd11b Enrichment Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd11b enrichment buffer/product/Miltenyi Biotec
    Average 99 stars, based on 17 article reviews
    cd11b enrichment buffer - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Isolation and Analysis of Mouse Microglial Cells"

    Article Title: Isolation and Analysis of Mouse Microglial Cells

    Journal: Current protocols in immunology / edited by John E. Coligan ... [et al.]

    doi: 10.1002/0471142735.im1435s104

    Common antibodies used for analysis of microglial cells by flow cytometry
    Figure Legend Snippet: Common antibodies used for analysis of microglial cells by flow cytometry

    Techniques Used:

    The total leukocyte population is gated from the raw data to exclude any debris that typically lies along the Y axis. It is imperative to only select the true leukocyte population in order to obtain clean plots. CD45 is sub gated from the all population into 3 different groups. CD45Hi population is made up of hematogenous derived cells that infiltrated the CNS, whereas the CD45lo population represents the CD11b+ microglial population. The CD45 negative population will be omitted from this analysis and likely contains astrocytes, or other glial and neuronal populations. Within the CD45Hi population, there are 2 populations of interest: infiltrating monocytes (CD11b+ CD11c−) and monocyte derived dendritic cells (CD11b+ CD11c+). From the CD45lo population graph CD11b by CD11c to determine the percentage of true resident microglia (CD11b+ CD11c−) and CD11b+ CD11c+ populations.
    Figure Legend Snippet: The total leukocyte population is gated from the raw data to exclude any debris that typically lies along the Y axis. It is imperative to only select the true leukocyte population in order to obtain clean plots. CD45 is sub gated from the all population into 3 different groups. CD45Hi population is made up of hematogenous derived cells that infiltrated the CNS, whereas the CD45lo population represents the CD11b+ microglial population. The CD45 negative population will be omitted from this analysis and likely contains astrocytes, or other glial and neuronal populations. Within the CD45Hi population, there are 2 populations of interest: infiltrating monocytes (CD11b+ CD11c−) and monocyte derived dendritic cells (CD11b+ CD11c+). From the CD45lo population graph CD11b by CD11c to determine the percentage of true resident microglia (CD11b+ CD11c−) and CD11b+ CD11c+ populations.

    Techniques Used: Derivative Assay



    Similar Products

    cd11b enrichment buffer  (Miltenyi Biotec)
    99
    Miltenyi Biotec cd11b enrichment buffer
    Common antibodies used for analysis of microglial cells by flow cytometry
    Cd11b Enrichment Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd11b enrichment buffer/product/Miltenyi Biotec
    Average 99 stars, based on 1 article reviews
    cd11b enrichment buffer - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Common antibodies used for analysis of microglial cells by flow cytometry

    Journal: Current protocols in immunology / edited by John E. Coligan ... [et al.]

    Article Title: Isolation and Analysis of Mouse Microglial Cells

    doi: 10.1002/0471142735.im1435s104

    Figure Lengend Snippet: Common antibodies used for analysis of microglial cells by flow cytometry

    Article Snippet: Myelin removal Buffer: Cold PBS containing 0.5% BSA (see Reagents and Solutions) Myelin Removal Beads II (Miltenyi Biotec, cat. No. 130-096-733) LS columns (Miltenyi Biotec, cat. No. 130-042-401) MS columns (Miltenyi Biotec, cat. No. 130-402-201) Sterile Transfer pipette 5mL polystyrene tubes 15 mL conical tubes MidiMACS Separator (Miltenyi Biotec, cat. No. 130-402-302) CD11b enrichment buffer (PBS, 0.5% BSA, 2mM EDTA) (see Reagents and Solutions) CD11b (Microglia) MicroBeads (Miltenyi Biotec, cat. No. 130-093-634) OctoMACS Separator (Miltenyi Biotec, cat. No.130-042-109)

    Techniques:

    The total leukocyte population is gated from the raw data to exclude any debris that typically lies along the Y axis. It is imperative to only select the true leukocyte population in order to obtain clean plots. CD45 is sub gated from the all population into 3 different groups. CD45Hi population is made up of hematogenous derived cells that infiltrated the CNS, whereas the CD45lo population represents the CD11b+ microglial population. The CD45 negative population will be omitted from this analysis and likely contains astrocytes, or other glial and neuronal populations. Within the CD45Hi population, there are 2 populations of interest: infiltrating monocytes (CD11b+ CD11c−) and monocyte derived dendritic cells (CD11b+ CD11c+). From the CD45lo population graph CD11b by CD11c to determine the percentage of true resident microglia (CD11b+ CD11c−) and CD11b+ CD11c+ populations.

    Journal: Current protocols in immunology / edited by John E. Coligan ... [et al.]

    Article Title: Isolation and Analysis of Mouse Microglial Cells

    doi: 10.1002/0471142735.im1435s104

    Figure Lengend Snippet: The total leukocyte population is gated from the raw data to exclude any debris that typically lies along the Y axis. It is imperative to only select the true leukocyte population in order to obtain clean plots. CD45 is sub gated from the all population into 3 different groups. CD45Hi population is made up of hematogenous derived cells that infiltrated the CNS, whereas the CD45lo population represents the CD11b+ microglial population. The CD45 negative population will be omitted from this analysis and likely contains astrocytes, or other glial and neuronal populations. Within the CD45Hi population, there are 2 populations of interest: infiltrating monocytes (CD11b+ CD11c−) and monocyte derived dendritic cells (CD11b+ CD11c+). From the CD45lo population graph CD11b by CD11c to determine the percentage of true resident microglia (CD11b+ CD11c−) and CD11b+ CD11c+ populations.

    Article Snippet: Myelin removal Buffer: Cold PBS containing 0.5% BSA (see Reagents and Solutions) Myelin Removal Beads II (Miltenyi Biotec, cat. No. 130-096-733) LS columns (Miltenyi Biotec, cat. No. 130-042-401) MS columns (Miltenyi Biotec, cat. No. 130-402-201) Sterile Transfer pipette 5mL polystyrene tubes 15 mL conical tubes MidiMACS Separator (Miltenyi Biotec, cat. No. 130-402-302) CD11b enrichment buffer (PBS, 0.5% BSA, 2mM EDTA) (see Reagents and Solutions) CD11b (Microglia) MicroBeads (Miltenyi Biotec, cat. No. 130-093-634) OctoMACS Separator (Miltenyi Biotec, cat. No.130-042-109)

    Techniques: Derivative Assay